McMaster Biophotonics Facility

Website is currently being transitioned. For information please visit:

www.facebook.com/mcmasterbiophotonicsfacility


General Information

Location and Contacts

MBF Location: Health Sciences Centre 4H14

MBF Contact: hungclk@mcmaster.ca, truantr@mcmaster.ca

Management and Policies

The MBF is currently run by Dr. Ray Truant. The MBF is heavily subsidized by the Truant lab (and Dr. David Andrews in the past). Recent changes have been made to make the facility more user-friendly and efficient. There is currently no grant support; if this changes, we will lower fees accordingly. Please read through our facility policy for more information.

Facility Keycard Access

Drop by HSC 4N53 to acquire a form (envelope on lab door), or download here

  • Fill out form and bring printed record of WHMIS safety training (from MOSAIC)

If you have an existing hospital access card – bring the 5 digits on the back

  • If you do not have one – follow instructions on the form
  • Speak to Claudia (in HSC 4N53) to acquire signatures

Bring completed form to Biochem Office (4N59)

User Etiquette

Any abuse of devices (i.e. dismantling without permission, damaging, abuse of internet usage etc). will result in access being revoked and service charges to restore the devices

Data is expected to be removed from devices as often as possible to allow other users to save data without the hard drives being full

Claudia facilitates as a tech as needed for technical support BUT IS NOT EXPECTED to be of service for all needs (EMAIL ONLY: hungclk@mcmaster.ca)

  • We will refuse access to devices/support if users are not respectful

FAQ


Training

  • Device training is done via video training (links are found with accompanying device information below)
  • This should allow users to train at their own pace and reduce efforts of personnel in the lab
  • THERE IS NO INDIVIDUAL IN-PERSON TRAINING EXCEPT FOR SPECIFIC SITUATIONS
  • Claudia facilitates as a tech as needed for technical support BUT IS NOT EXPECTED to be of service for all needs (EMAIL ONLY: hungclk@mcmaster.ca)

Basic Microscopy Resources


Booking

All time booked will be charged, unless cancelled at least 24 hours before booking time. Accommodations can be made to switch times ONLY if approved by Claudia. Booking is done below on calendar. Invoice will be sent to email account provided.

https://raytruantlab.ca/mcmaster-biophotonics-facility-booking/


Devices

EVOS FL Auto 2 Microscope

3663210762001_5493650656001_5493447330001-vs

  • Widefield, inverted scope
  • Multichannel imaging, Z-stacks, plate screening, image stitching, time-lapse
  • Live cell imaging (heated stage and gas control)
  • 5 available channels at once out of this selection (make note in your booking) 
  • Transmitted, DAPI, CFP, YFP, RFP, TxRed, Cy5
  • Internal User Price: $20/hour
  • External User Price: $40/hour
  • Minimum booking time: 1 hour

TECAN M1000 Fluorescent Plate Reader

download

  • Luminescence and Fluorescence
    • Internal User Price: $20/hour
    • External User Price: $40/hour
  • Minimum booking time: 5 minutes

Leica SP5 Confocal Microscope

csm_TCS_SP5_1_0928363c21

  • Laser scanning confocal
    • Internal User Price: $40/hour
  • External User Price: $100/hour
  • Minimum booking time = 1 hour
  • Training: Video and in-person supervised practice (both needed to make bookings)
    • 2. Book a supervised practice by emailing hungclk@mcmaster.ca

Nikon SR-SIM Microscope